The aim of this study was to determine the cross-sectional area (CSA) of the peroneus brevis, the peroneus longus, and connective tissue; and to compare these measurements in participants with ankle sprains, and in those without ankle sprains.
The intraexaminer reliability of the ultrasonography protocol used to quantify these measures was also assessed.
A sample of 56 feet was obtained from medical records at the CARMASALUD clinical and research centre in Madrid, Spain (26 feet with a prior grade I or II diagnosis of lateral ankle sprain (LAS) and 26 feet without this condition).
Ultrasound imaging was performed with the participants in the supine decubitus position and the ankle placed at 90 degrees of dorsal flexion with respect to the frontal plane, using a wedge to avoid the bias of muscle activity during rehabilitative ultrasound imaging (RUSI) evaluation.
The transducer was positioned perpendicular to the peroneal bone, with the midpoint of the transducer footprint coinciding with the midpoint of the width of the peroneus muscles.
Before still images and measurements were acquired, a dynamic evaluation protocol was carried out to assess the limits between the peroneus brevis and the peroneus longus by means of transducer movement along the proximal area of the peroneus muscles.
In the current study, larger CSAs were obtained for feet without LAS and feet with LAS by using measurements taken at the same point because the total area of the peroneus brevis and the peroneus longus was measured by using a border outside connective tissue.
The peroneus longus CSA was found to be reduced in participants with LAS compared with that in participants without LAS.
The intraexaminer reliability of the ultrasound protocol used to quantify the peroneus brevis and the peroneus longus muscle tissues was excellent, and the protocol was acceptable in the case of connective tissue.
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